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| Li, H; Parisi, M; Toubiana, Mylene; Cammarata, M; Roch, Philippe. |
| The aim of the present study was to evaluate the expression of the Mytilus galloprovincialis lysozyme gene in different in vivo stress situations, including injection of bacteria Vibrio splendidus LGP32, Vibrio anguillarum or Micrococcus lysodeikticus, as well as heat shock at 30 C and cold stress at 5 C. Injection of V. splendidus LGP32 resulted in: (i) a general down-regulation of lysozyme gene expression, as quantified by Q-PCR; (ii) reduction in the number of circulating hemocytes; (iii) decrease in the percentage of circulating hemocytes expressing lysozyme mRNA which was now restricted to only small cells, as observed by ISH; and (iv) accumulation of hemocytes expressing lysozyme in the muscle sinus where injection took place. Injection of V.... |
| Tipo: Text |
Palavras-chave: Molluscs; Mytilus; ISH; Q PCR; Gene expression; Lysozyme; Heat shock; Micrococcus lysodeikticus; Vibrio anguillarum; Vibrio splendidus. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4479.pdf |
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| Catois, Stéphane; Haure, Joel; Martin, J-l.; Nourry, Max; Palvadeau, Hubert; Baud, Jean-pierre. |
| Mortality among juvenile Crassostrea gigas has been occuring during the summer months since 1995 and is now considered to be a real problem for French oyster culture. The aim of this study was to standardise the stress that could be applied to Crassostrea gigas spat. In initial experiments in 1998, the weight of individual spat and the duration of a heat shock at 50°C in freshwater, adapted for oysters of between 500 and 2000 mg, were built into a model to obtain 50% mortality. However, the precision was low (50%) and will need to be improved in the future. Following this, hatchery-produced juveniles weighing 10 to 500 mg were subjected to heat shock at 45°C in heshwater ; the duration of the shock was calculated based on a model built in 1997. The results... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Stress; Choc thermique; Mortalité; Crassostrea gigas; Stress; Heat shock; Mortality. |
| Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/00080/19095/16690.pdf |
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| Heng,Boon Chin; Vinoth,Kumar Jayaseelan; Lu,Kai; Deng,Xuliang; Ge,Zigang; Bay,Boon Huat; Cao,Tong. |
| We investigated the effects of prolonged heat shock treatment on human embryonic stem cell (hESC) viability. The hESC viability steadily declined with longer exposure to heat shock treatment (43ºC). After 4 h of exposure to heat shock at 43ºC, only 56.2 ± 1.5% of cells were viable. Viability subsequently declined to 37.0 ± 3.3% and 3.5 ± 0.7% after 8 h and 16 h, respectively of heat shock treatment at 43ºC. Transmission electron micrographs showed that the morphology of the dead/dying cells after heat shock treatment was characteristic of cellular necrosis with an uncondensed chromatin and a non-intact plasma membrane. This was further confirmed by flow cytometry analysis which showed that the DNA of the dead/ dying cells was still mostly intact, unlike... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Embryonic; Heat shock; Human; Necrosis; Stem cells. |
| Ano: 2007 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000300007 |
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| Hernandez,Pedro P; Undurraga,Cristian; Gallardo,Viviana E; Mackenzie,Natalia; Allende,Miguel L; Reyes,Ariel E. |
| Copper is an essential ion that forms part of the active sites of many proteins. At the same time, an excess of this metal produces free radicals that are toxic for cells and organisms. Fish have been used extensively to study the effects of metals, including copper, present in food or the environment. It has been shown that different metals induce different adaptive responses in adult fish. However, until now, scant information has been available about the responses that are induced by waterborne copper during early life stages of fish. Here, acute toxicity tests and LC50 curves have been generated for zebrafish larvae exposed to dissolved copper sulphate at different concentrations and for different treatment times. We determined that the larvae... |
| Tipo: Journal article |
Palavras-chave: Acute exposure; Apoptosis; Copper; Early life stage; Heat shock; Protein-70; Zebrafish. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000100002 |
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